Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
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چکیده
منابع مشابه
Chromatography of Myoglobin on Diethylaminoethyl Cellulose Columns*
Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the ide...
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Thyroglobulin may be defined as the iodine-containing protein of the thyroid gland which has a sedimentation coefficient of 4o.w = 19 S, an electrophoretic mobility at pH 8.6 of approximately 5 x 10F5 cm2 volt-l set-1, and a steep salting out curve at about 38% ammonium sulfate saturation at neutral pH (1). Although other iodoproteins, both soluble in neutral salt and attached to cell particles...
متن کاملThe preparation and purification of individual human pepsins by using diethylaminoethyl-cellulose.
A procedure was devised for isolating human pepsins 1, 2, 3 and 5 from gastric juice by repetitive column chromatography on DEAE-cellulose. The combined yields in four different experiments varied from 14% to 90% of the total peptic activity of the starting material. The isolated individual pepsins were shown to behave as single homogeneous proteins on agar-gel electrophoresis at pH 5.0 and on ...
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The serum proteins designated as y-globulins include all proteins of the slowest moving of the several major protein groups seen on serum electrophoresis conducted at alkaline pH (2). Among the y-globulins of normal serum are proteins of differing electrophoretic mobility (3,4), proteins with sedimentation coefficients of 6.6 S and 18 S in the ultracentrifuge (2, 3, 5, 6)) many individual antib...
متن کاملPurification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2019
ISSN: 1940-087X
DOI: 10.3791/58415